Journal: Molecular biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion.

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Fig. 2 Effect of FGF2 deficiency on BMDM apoptosis and polarization. a–c FGF2 deletion increased BMDM apoptosis. a Apoptosis in BMDM deprived of FBS for 24 h was assessed by flow cytometry (n = 4). b-c Percentage of PI + Annexin V + and PI- Annexin V + BMDM after starvation. d-k FGF2 deletion in BMDM promoted M1 polarization. d-g Flow cytometric analysis of macrophage markers in BMDM treated with LPS or IL4, including CD86, iNOS, CD206, and Arg1 (n = 3). h-k The levels of CD86, iNOS, CD206 and Arg1 in BMDM after treatment with LPS or IL4. N represents no treatment; * p < 0.05, vs. WT; Ψ p < 0.05, vs. N + WT; Ω p < 0.05, vs. N + FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Flow Cytometry

Journal: Molecular biomedicine

Article Title: Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion.

doi: 10.1186/s43556-024-00203-0

Figure Lengend Snippet: Fig. 6 Mice reconstituted with FGF2 KO macrophages and subjected to CLP demonstrate increased M1 polarization in lung tissue. a-f The presence and levels of CD206, CD86, and F4/80 markers on macrophages within lung tissue were identified and quantitatively assessed using immunofluorescence staining. Bar is 20 μm. * p < 0.05, vs. WT; Δ p < 0.05 vs. WT + LPS; # p < 0.05 vs. FGF2 KO

Article Snippet: The tissue sections were incubated overnight at 4°C with primary antibodies, including the rabbit polyclonal antibody CD206 (1:200, Cat No. A02285-2, Boster, Wuhan, China), rabbit monoclonal antibody CD86 (1:100, Cat No. BM4121, Boster, Wuhan, China), rabbit polyclonal antibody F4/80 (1:100, Cat No. 29414-1-AP, Proteintech, Wuhan, China), anti-mouse NLRP3 (1:200, Cat No.68102-1-Ig, Proteintech, Wuhan, China), Caspase-1 p20 Rabbit pAb (1:400, Cat No.bs-10743R, Bioss, Beijing, China) and ASC/TMS1 Rabbit PolyAb (1:200, Cat No.10500-1-AP, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining

Journal: Materials Today Bio

Article Title: Hyperthermia/glutathione-triggered ferritin nanoparticles amplify the ferroptosis for synergistic tumor therapy

doi: 10.1016/j.mtbio.2024.101085

Figure Lengend Snippet: The mechanisms underlying occurrence of ferroptosis. (A) A schematic illustration of GSH-triggered and PTT-enhanced ferroptosis. (B) GSH contents normalized to the total protein concentration in MCF-7/ADR cells after different treatments ( n = 5). (C) Cellular protein expression of GPX-4 in MCF-7/ADR cells after treatments with I@P and I@P-ss-FRT (50 μg Fe/mL) with or without 808 nm laser exposure (1.25 W/cm 2 , 5 min) and (D) the relative level of GPX-4/β-actin ( n = 3). (E) CLSM images of MCF-7/ADR cells after incubation with I@P and I@P-ss-FRT showing intracellular Fe 2+ ions levels using fluorescent probe FerroOrange and (F) the quantification of fluorescence intensity inside cells; Scale bar = 50 μm ( n = 3). (G) CLSM images, (H) mean fluorescent intensity of the cells determined from the CLSM images, and (I) FCM analysis of ROS production in MCF-7/ADR cells after different treatments; Scale bar = 50 μm ( n = 3).

Article Snippet: Cell lysis buffer, phenylmethanesulfonylfluoride (PMSF), SDS-PAGE sample loading buffer, nonfat powdered milk, HRP-labeled goat anti-mouse IgG (H + L), HRP-labeled goat anti-rabbit IgG (H + L), BeyoECL Plus, BCA protein assay kit, Calcein/PI cell viability/cytotoxicity assay kit, reactive oxygen species (ROS) assay kit, mannose receptor C-type 1 (MRC1) rabbit polyclonal antibody and β-actin mouse monoclonal antibody were bought from Beyotime Biotechnology Co., Ltd (Shanghai, China).

Techniques: Protein Concentration, Expressing, Incubation, Fluorescence